Rapid detection method of waxy phenotype in sorghum seed and application thereof

The invention relates to waxy phenotype identification of crop grains, in particular to a method for quickly detecting waxy phenotype of sorghum seeds and application thereof. The method comprises the following steps: placing a cell culture plate on a firm horizontal table, placing a sorghum seed sample in each hole, and mashing the seed in the hole with a miniature pestle; Add water at 90-100°C to the hole containing the mashed sample, 0.3ml-0.5ml per hole; after the liquid in the holes is cooled to room temperature, add a drop of iodine staining solution to each hole; If dyed blue-black, the sample is non-waxy sorghum; If dyed brown-red, orange or light yellow, the sample is waxy sorghum. The method can quickly and accurately distinguish waxy sorghum and non-waxy sorghum seeds without peeling the seeds, using a pulverizer, a water bath pot or an oven. Figure 3 Figure 4