PREPARATION METHOD OF SPVD RECOMBINANT DOUBLE CP FUSION PROTEIN AND METHOD FOR DETECTING SPVD

PREPARATION METHOD OF SPVD RECOMBINANT DOUBLE CP FUSION PROTEIN AND METHOD FOR DETECTING SPVD

The present invention belongs to the field of biotechnology, and disclosing a preparation method of a sweet potato virus disease (SPVD) recombinant double CP fusion protein and a method for detecting SPVD. The SPVD recombinant double CP fusion protein is constructed from the fusion fragment of the C...

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Bibliographische Detailangaben
Hauptverfasser: XIANG, JIE, QIAO, LIXIAN, LI, QIYAN, GENG, QICHAO, MA, TAO, ZHU, HONG, ZHAO, CHUNMEI, DU, GUONING, TANG, YANYAN, SUI, JIONGMING, LIN, SHUNYU, WANG, JINGSHAN
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention belongs to the field of biotechnology, and disclosing a preparation method of a sweet potato virus disease (SPVD) recombinant double CP fusion protein and a method for detecting SPVD. The SPVD recombinant double CP fusion protein is constructed from the fusion fragment of the CP gene of sweet potato greenish dwarfing virus and sweet potato plume mottled virus to the recombinant expression vector, transformed into the expression strain and induced expression. The existing method for detecting SPVD can only detect sweet potato chlorotic stunt virus and sweet potato feathery mottle virus separately, resulting in low detection efficiency and high cost. By preparing the SPVD recombinant double CP polyclonal antibody, sweet potato chlorotic stunt virus and feathery mottle virus can be simultaneously detected, which improves detection efficiency and reduces detection cost.