A duck plague virus UL41 gene markerless deletion strain CHv-BAC-G-ΔUL41 and a construction method thereof

The present invention discloses a duck plague virus UL41 gene markerless deletion strain CHv BAC-G-AUL41 and a construction method thereof, belonging to the technical field of biology. According to the invention, GS1783 Escherichia coli strain and pEPKan-S plasmid are utilized to delete the UL41 gen...

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Bibliographische Detailangaben
Hauptverfasser:	Cheng, Anchun, Wang, Mingshu, He, Tianqiong
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention discloses a duck plague virus UL41 gene markerless deletion strain CHv BAC-G-AUL41 and a construction method thereof, belonging to the technical field of biology. According to the invention, GS1783 Escherichia coli strain and pEPKan-S plasmid are utilized to delete the UL41 gene of duck plague virus through two-step homologous recombination on the platform of a bacterial artificial chromosome recombination duck plague virus rescue system, and the construction of duck plague virus markerless deletion strain without exogenous base residue is completed for the first time. According to the invention, the problem of residual base at the deletion site when the duck plague virus gene is deleted is solved, and sufficient technical support is provided for accurately exploring the function of duck plague virus gene and constructing an attenuated live vaccine. -1/4 pEPkan-S 6288 bp -I (~ 17romoter CCAP binding sitel v (iac opraorr Fig. 1