DNA-cutting agent based on Cas9 protein from the bacterium Pasteurella pneumotropica

The present invention describes a novel bacterial nuclease of a CRISPR-Cas9 system from the bacterium P. pneumotropica, as well as the use of said nuclease for creating strictly specific two-strand cuts in a DNA molecule. The present nuclease possesses unusual properties and can be used as an instru...

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Bibliographische Detailangaben
Hauptverfasser:	ARTAMONOVA, Daria Nikolaevna, FEDOROVA, Yana Vitalievna, ARTAMONOVA, Tatiana Olegovna, ANDREEVA, Julia Valerevna, KHODORKOVSKII, Mikhail Alekseevich, VASILIEVA, Aleksandra Andreevna, GORYANIN, Ignaty Igorevich, SELKOVA, Polina Anatolevna, SEVERINOV, Konstantin Viktorovich, SHMAKOV, Sergey Anatolievich, POBEGALOV, George Evgenevich, ARSENIEV, Anatoliy Nikolaevich, MUSHAROVA, Olga Sergeevna, ZYUBKO, Tatiana Igorevna, ABRAMOVA, Marina Viktorovna
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention describes a novel bacterial nuclease of a CRISPR-Cas9 system from the bacterium P. pneumotropica, as well as the use of said nuclease for creating strictly specific two-strand cuts in a DNA molecule. The present nuclease possesses unusual properties and can be used as an instrument for introducing changes at strictly specified locations in a genomic DNA sequence of single-celled and multi-celled organisms. The invention thus increases the universality of accessible CRISPR-Cas9 systems, making it possible to use Cas9 nuclease from various organisms to cut genomic or plasmid DNA in a large number of specific sites and under various conditions.