standard gene database and molecular identification method for molecular identification of Coptis chinensis

Abstract The invention discloses a standard gene database for molecular identification of Coptis. The nucleotide sequence is the sequence in the sequence table, including the ycfl sequence matrix of Coptis plants, which contains 19 species of coptis plants known worldwide. The molecular identification method includes: extracting total DNA from sample; PCR amplification of ycfl gene fragment; splicing of ycfl sequence sequencing results to obtain ycfl base According to the NJ tree, we constructed the NJ tree of ycfl sequence. The advantages of the invention are as follows: the specific primer pairs suitable for the amplification of ycfl sequence of Coptis plants are designed, and the target sequence can be obtained efficiently; whether the counterfeit Coptis comes from home or abroad, it can be accurately identified; the identification efficiency of traditional method in Coptis is only 20% - 40%, while the ycfl sequence identification efficiency of the invention is more than 95%, which greatly improves the score of Coptis chinensis The success rate of sub identification.