Methods for differentiating pluripotent stem cells in dynamic suspension culture

Methods for differentiating pluripotent stem cells in dynamic suspension culture

Methods for differentiating pluripotent stem cells to neuroectoderm in dynamic suspension culture using small molecule or protein inhibitors of TGFβ/Activin/Nodal signaling and BMP signaling are provided. Also provided are methot and protocols for differentiating pluripotent stem cells such as human...

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Hauptverfasser: NAIR, Rekha R, SHOUKAT-MUMTAZ, Uzma, HALBERSTADT, Craig R, WHITELEY, Erik Michael, KAYSER, Stephanie, PARIKH, Abhirath S, MANLEY, Nathan C
Format: Patent
Sprache:eng
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BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
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MICROBIOLOGY
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Zusammenfassung:Methods for differentiating pluripotent stem cells to neuroectoderm in dynamic suspension culture using small molecule or protein inhibitors of TGFβ/Activin/Nodal signaling and BMP signaling are provided. Also provided are methot and protocols for differentiating pluripotent stem cells such as human embryonic stem cells first to neuroectoderm, then further to glial progenitor cells, and further to oligodendrocyte progenitor cells (OPCs), and compositions obtained thereby. The methods of the present disclosure reproducibly produce neuroectoderm progenitor cells by day 7 of the differentiation process, glial progenitor cells by day 21 of the differentiation process and OPCs by day 42 of the differentiation process.