Inducible expression system for plasmid-free production of a protein of interest

Inducible expression system for plasmid-free production of a protein of interest

A genome-based expression system for production of a protein of interest (POI) in a prokaryotic host, comprising at least an RNA polymerase (RNAP) gene, a gene encoding a POI, comprising a coding sequence, a promoter operably linked to said coding sequence, wherein said promoter is recognized by the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: CSERJAN, Monika, STRIEDNER, Gerald, GRABHERR, Reingard, SCHULLER, Artur
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A genome-based expression system for production of a protein of interest (POI) in a prokaryotic host, comprising at least an RNA polymerase (RNAP) gene, a gene encoding a POI, comprising a coding sequence, a promoter operably linked to said coding sequence, wherein said promoter is recognized by the RNAP expressed from the RNAP gene, and at least one lac operator (lacO) within the sequence of said promoter; and a lacI gene encoding a lac repressor protein (LacI) comprising a coding sequence, a lacI promoter operably linked to the lacI coding sequence, wherein the lacI promoter is a wild-type lacI promoter or a lacI promoter which increases LacI expression; wherein the expression rate of the POI is regulated by an inducer binding LacI.