Methods for gene modification of hematopoietic cells

The present invention relates generally to methods for genetic modification of hematopoietic cells. In particular, the invention relates to use of Prostaglandin E2 (PGE2), poloxamer, and protamine sulfate to enhance transduction by a recombinant retroviral vector. The compositions and methods of the...

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Bibliographische Detailangaben
Hauptverfasser:	SHAH, Gaurav D, RIO GALDO, Paula, SEGOVIA SANZ, Jose Carlos, QUINTANA BUSTAMANTE, Oscar, MESA NUNEZ, Cristina, BUEREN RONCERO, Juan Antonio, BEARD, Brian, ALMARZA NOVOA, Elena, LAW, Kenneth, PATEL, Kinnari, NAVARRO ORDONEZ, Susana
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates generally to methods for genetic modification of hematopoietic cells. In particular, the invention relates to use of Prostaglandin E2 (PGE2), poloxamer, and protamine sulfate to enhance transduction by a recombinant retroviral vector. The compositions and methods of the present disclosure are particularly suitable for gene therapy applications, including the treatment of monogenic genetic diseases and disorders.