HEAT TREATMENT TO PRODUCE GLYCOSIDES

Abstract The present invention relates to methods of efficiently recovering desired steviol glycosides produced from within engineered host cells such as yeast. The methods involve heat treatments which release desired glycosides from host cells with minimal lysis of the cells thereby enriching the...

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Bibliographische Detailangaben
Hauptverfasser:	Flores, Belit, Rasmussen, Maribeth, Paulson, Nicole, Nygaard, Richard, Brower III, Robert J, Mortenson, Kristopher T, Gaspard, Dan S, Carlson, Ting Liu, Anderson, James C
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	Abstract The present invention relates to methods of efficiently recovering desired steviol glycosides produced from within engineered host cells such as yeast. The methods involve heat treatments which release desired glycosides from host cells with minimal lysis of the cells thereby enriching the fermentation media or composition for the desired glycoside. WO 2016/196345 PCT/US2016/034781 HO SCoA 411(¾()c'Ax4tfhasgo o OH til 1 0 fi e -'l W It ph5sphomeraplae 0 -P--P OH aknwpyrophphateO~ '0 0 oP-O-P-OH dimthydy9pyrophosphate (t hAPP}