Bordetella pertussis-derived protein-obtaining method including affinity chromatography process

Bordetella pertussis-derived protein-obtaining method including affinity chromatography process

The present invention relates to a Bordetella pertussis-derived protein-obtaining method including an affinity chromatography process. The present invention increases the production yield of PT and FHA proteins by isolating PT and FHA proteins of Bordetella pertussis through a purification process u...

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Hauptverfasser: KIM, Hae Ryun, JEON, Hyung Jin, CHOI, Gi Sub, PARK, Jong Kwan, AHN, Dong Ho, CHOI, Bo Mi, MOON, Jae Hoon
Format: Patent
Sprache:eng
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CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
TRANSPORTING
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creator KIM, Hae Ryun
JEON, Hyung Jin
CHOI, Gi Sub
PARK, Jong Kwan
AHN, Dong Ho
CHOI, Bo Mi
MOON, Jae Hoon
description The present invention relates to a Bordetella pertussis-derived protein-obtaining method including an affinity chromatography process. The present invention increases the production yield of PT and FHA proteins by isolating PT and FHA proteins of Bordetella pertussis through a purification process using a blue affinity column. In addition, when used, the filtration process can remarkably increase the output of the target proteins, compared to an isolation method using a size exclusion chromatography (SEC) process. Furthermore, the method using the blue affinity column for isolation of PT and FHA proteins decreased resin expense, process time, buffer consumption, and production cost and significantly increased the productivity of PT and FHA proteins.
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subjects CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
TRANSPORTING
title Bordetella pertussis-derived protein-obtaining method including affinity chromatography process
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