Bordetella pertussis-derived protein-obtaining method including affinity chromatography process

The present invention relates to a Bordetella pertussis-derived protein-obtaining method including an affinity chromatography process. The present invention increases the production yield of PT and FHA proteins by isolating PT and FHA proteins of Bordetella pertussis through a purification process using a blue affinity column. In addition, when used, the filtration process can remarkably increase the output of the target proteins, compared to an isolation method using a size exclusion chromatography (SEC) process. Furthermore, the method using the blue affinity column for isolation of PT and FHA proteins decreased resin expense, process time, buffer consumption, and production cost and significantly increased the productivity of PT and FHA proteins.