Protease modulating wound interface layer for use with negative pressure wound therapy

Systems, methods, and apparatuses for modulating proteases including matrix metalloproteinase (MMP), elastase, and bacterial protease in a negative pressure therapy system are described. A mesh having a sacrificial substrate is included. The sacrificial substrate includes a plurality of collagen fibers reinforced with a supporting material and intersecting with each other to form a network of collagen fibers having a plurality openings. The openings of the plurality of openings have an average area between about 0.5 mm2 and about 20 mm 2 to permit the flow of negative pressure through the mesh. The sacrificial substrate can also include oxidized regenerated cellulose. C) Cal cc C) C'4 C) co c) C) C*4 C) 'D C%4 C) C*4