Oligonucleotide and nucleic acid synthesis

Oligonucleotide and nucleic acid synthesis

The present invention relates to methods for the high fidelity synthesis of oligonucleotides and polynucleotides on a solid surface. In particular, the invention relates to methods of synthesising oligonucleotides, polynucleotides, and doublestranded polynucleotides/nucleic acids, such as DNA and XN...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: CROSBY, Stuart, BARLOW, Iain, LANG, Pascal, FITZPATRICK, Catherine, HAYES, Matthew, BRENNAN, Joseph, BYGRAVE, Daniel, JENISON, Mathew
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
COMBINATORIAL CHEMISTRY
DERIVATIVES THEREOF
LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SUGARS
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Beschreibung
Zusammenfassung:The present invention relates to methods for the high fidelity synthesis of oligonucleotides and polynucleotides on a solid surface. In particular, the invention relates to methods of synthesising oligonucleotides, polynucleotides, and doublestranded polynucleotides/nucleic acids, such as DNA and XNA, wherein the process comprises thermally controlled deprotection steps at the 5'-OH of previously coupled nucleosides or nucleotides at selected sites on the surface of the substrate.