Bidirectional CHEFl vectors

Bidirectional CHEFl vectors

The invention provides bidirectional expression vectors comprising Chinese hamster ovary elongation factor 1-a (CHEFl) transcriptional regulatory DNA elements, a gene of interest (GO I), a minimal cytomegalovirus (minCMV) and a selectable marker (SM) and/or a human adenovirus tripartite leader (AdTP...

Ausführliche Beschreibung

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Bibliographische Detailangaben
1. Verfasser: PANDE, Sandhya
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention provides bidirectional expression vectors comprising Chinese hamster ovary elongation factor 1-a (CHEFl) transcriptional regulatory DNA elements, a gene of interest (GO I), a minimal cytomegalovirus (minCMV) and a selectable marker (SM) and/or a human adenovirus tripartite leader (AdTPL) sequence. The invention also provides method for increasing heterologous protein expression in a host cell comprising culturing the host cell the bidirectional expression vector(s).