Biological fluid collection device

A biological fluid collection device (10), comprising: a housing (13) having a superior surface (14) and an inferior surface (16), wherein a portion of the superior surface defines a cavity having a cavity superior surface; a film engageable with the housing, the film having a film inferior surface and a film superior surface, the film transitionable between an initial position in which the film inferior surface is in contact with the cavity superior surface and a fill position in which the film inferior surface is spaced from the cavity superior surface forming a chamber between the film and the housing; and an actuator (24) disposed at least partially within the housing and in communication with the film and the housing, the actuator transitionable between an original position and a depressed position, wherein after actuation of the actuator to the depressed position, as the actuator returns to the original position, the actuator applies a vacuum to the film superior surface.