Improved AAV capsid production in insect cells

The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno- associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is...

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Bibliographische Detailangaben
Hauptverfasser:	BOSMA, Sebastiaan Menno, PETRY, Harald Peter Albert, LUBELSKI, Jacek, DU PLESSIS, David Johannes Francois, TER BRAKE, Olivier
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno- associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is an AUG. Upstream of the VP1 open reading frame an alternative out of frame start codon is placed such that translation initiation of the VP1 protein is modified, i.e. reduced, to allow production of VP1 :VP2: VP3 in a good stoichiometry resulting in AAV with high potency.