Method for evaluating differentiation state of cells

To provide a method whereby pluripotent stem cells can be detected at a high sensitivity without disrupting the cells after inducing differentiation. The present invention provides a method which comprises measuring miRNA in miR302/367 cluster in a liquid phase fraction of a cell culture liquid duri...

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Bibliographische Detailangaben
1. Verfasser:	Aihara, Yuki
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	To provide a method whereby pluripotent stem cells can be detected at a high sensitivity without disrupting the cells after inducing differentiation. The present invention provides a method which comprises measuring miRNA in miR302/367 cluster in a liquid phase fraction of a cell culture liquid during and/or after inducing differentiation of pluripotent stem cells and evaluating the differentiation state of the cells in the cell culture liquid on the basis of the measurement value of miRNA. The present invention also provides a method for detecting pluripotent stem cells, said method comprising a step for measuring miRNA in miR302/367 cluster in a liquid phase fraction of a cell culture liquid during and/or after inducing differentiation of pluripotent stem cells and a step for detecting the pluripotent stem cells in the cell culture liquid on the basis of the measurement value of miRNA.