Methods for chemical ligation of nucleic acids

Provided herein are methods for preparing nucleic acid libraries, capturing DNA obtained from a limited number of cells, and selectively cleaving ssDNA or dsDNA using various chemical ligation and click chemistry reactions described herein.

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Bibliographische Detailangaben
Hauptverfasser: Chen, Xi-Jun, Khurana, Tarun, Teo, Yin Nah
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Provided herein are methods for preparing nucleic acid libraries, capturing DNA obtained from a limited number of cells, and selectively cleaving ssDNA or dsDNA using various chemical ligation and click chemistry reactions described herein.