Methods for directed differentiation of pluripotent stem cells to HLA homozygous immune cells

Methods for directed differentiation of pluripotent stem cells to HLA homozygous immune cells

Provided herein are methods for the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid li...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Rajesh, Deepika, Wang, Wen Bo, Zhang, Xin, Munn, Christie, Brandl, Andrew J, Vodyanyk, Maksym A, Burton, Sarah, Swanson, Bradley
Format: Patent
Sprache:eng
Schlagworte:
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BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
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Beschreibung
Zusammenfassung:Provided herein are methods for the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.