Integrated Sequencing Apparatuses And Methods Of Use

Provided are methods and apparatuses for performing sequencing using electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same 5 apparatus. In addition, provided are methods of reducing reagent waste and preload...

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Hauptverfasser:	Gong, Jian, Sakaldasis, George, Vermaas, Eric, Bohm, Sebastian, Shen, Min-Jui Richard, Kain, Robert C, Khurana, Tarun, Talasaz, Amirali Hajhossein, Jamshidi, Arash, Kuhn, Kenneth M, Eltoukhy, Helmy A
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL TRANSPORTING
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Beschreibung
Zusammenfassung:	Provided are methods and apparatuses for performing sequencing using electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same 5 apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing. WO 2013/070627 PCT/US20 12/063741 Electrodes Liquid flow -l D Substrate mole -~A --- detection pixels Fig. 1 Electrodes Top of flowcell Flowcell Liquid fDNA Substrate molecules --- CMOS chip ---. ./... --- detection pixeVs Fig. 2 Fig. 3