Method for preparing a chitosan-based matrix comprising a fiber reinforcement member

Method for preparing a chitosan-based matrix comprising a fiber reinforcement member The present invention relates to a method for preparing a chitosan-based matrix (4) comprising the following steps: - a) two separate chitosan porous layers (1, 2) obtained from lyophilisation of layers of one or more solutions of chitosan are provided, - b) the chitosan porous layers of a) are neutralized and washed, - ) a face (1a, 2a) of each chitosan porous layer is acidified with an acid, - d) a fiber reinforcement member (3) is provided and positioned between the two acidified faces of the two chitosan porous layers, - e) the two chitosan porous layers and the fiber reinforcement member are let to dry to obtain a matrix having the fiber reinforcement member embedded therein. The invention also relates to an implant comprising a matrix obtained according to such a method.