Method for enriching target nucleic acid sequence from nucleic acid sample

The present disclosure provides a method for enriching the nucleic acids of target sequences from a nucleic acid sample, the method comprising: providing a nucleic acid sample comprising the target nucleic acid sequences and bait sequences that are identical to or specific to the sequences of the target nucleic acids; performing in vitro transcription with the bait sequences as templates to prepare nucleic acid analogs, where the nucleic acid analogs having a binding moiety; fragmenting the nucleic acid sample; hybridizing said nucleic acid analogs to said nucleic acid sample, such that said nucleic acid analogs form nucleic acid analog/DNA hybrid complexes with said target sequence nucleic acids; isolating the nucleic acid analog/DNA hybrid complexes from non specific hybrid nucleic acids by the binding moieties, to remove the non-target sequence nucleic acids. In a preferred embodiment, the method further comprises: amplifying the nucleic acid analog/DNA hybrid complexes, so as to enriching the nucleic acids of the target sequences.