Transposase compositions for reduction of insertion bias

Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

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Bibliographische Detailangaben
Hauptverfasser: Osothprarop, Trina Faye, Jendrisak, Jerome, He, Molly, Amini, Sasan, Gunderson, Kevin L, Kia, Amirali, Steemers, Frank J, Gloeckner, Christian
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.