High-throughput DNA fragment assembly

High-throughput DNA fragment assembly

This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96- well plates. In some embodiments,...

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Bibliographische Detailangaben
Hauptverfasser: Evans, Steven L, Kumar, Sandeep, Gupta, Manju
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96- well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.