Precise breeding

The present invention relates to a method for identifying and isolating native plant nucleic acid sequences that may function as T-DNAs or T-DNA border-like sequences, effecting the transfer of one polynucleotide into another polynucleotide. The present invention also provides a modified tuber, such...

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Bibliographische Detailangaben
Hauptverfasser: ROMMENS, CAIUS, MENENDEZ-HUMARA, JAIME, YAN, HUA, YE, JINGSONG, SWORDS, KATHY
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention relates to a method for identifying and isolating native plant nucleic acid sequences that may function as T-DNAs or T-DNA border-like sequences, effecting the transfer of one polynucleotide into another polynucleotide. The present invention also provides a modified tuber, such as a genetically modified mature tuber, that comprises at least one trait that is not exhibited by a non-modified tuber of the same species. WO 2005/004585 PCT/US2004/017424 Figure 1. Diagrams for some P-DNA vectors 1 416 1 2335 pSIM1 11 .pSIM108 646-9,323-bp 80-11,251-bp 1 3114 4937 1 4970 :mPPO =INH' GUS 13613 147-* pSIM340 .pSIM356 1 6 13,853-bp 11880 14727-bp 1 4558 1 7602 Lp2 Lph 1627 Lpo LPH Alf pSIM70 .pSIM371 pSIM370 4 16,5 18-bp 13,474-bp 10627-- 13I71