Sample preparation for flow cytometry

Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-re...

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Bibliographische Detailangaben
Hauptverfasser: Yup, Axel A, Kopher, Kenneth Anthony, Shi, Song, Li, Xiao, Mantlo, John D, Pope, William Alfred, Zhang, Yongqiang
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.