Expression of meningococcal fhbp polypeptides

The meningococcal Jhbp gene (encoding factor H binding protein) is naturally expressed from two independent transcripts by two differentially regulated promoters. In one transcript it is co-expressed 5 with the neighbouring upstream gene from the Pnmnb869 promoter. The other transcript is monocistro...

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Hauptverfasser:	ORIENTE, FRANCESCA, DELANY, ISABEL
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The meningococcal Jhbp gene (encoding factor H binding protein) is naturally expressed from two independent transcripts by two differentially regulated promoters. In one transcript it is co-expressed 5 with the neighbouring upstream gene from the Pnmnb869 promoter. The other transcript is monocistronic and is expressed from its own dedicated promoter, Pfrbp, which is activated by the global regulatory protein FNR in response to oxygen-limiting conditions. To increase expression of the monocistronic transcript a constitutively-active FNR mutant is used. The Pfrbp promoter can thus be activated, leading to over-expression of FNR-activated genes, such asfhbp.