Method for the continuous production of cellulases by a filamentous fungus using a carbon substrate obtained from an acid pretreatment
The present invention relates to a method for producing cellulases and hemicellulases by a strain belonging to a filamentous fungus, in an agitated and aerated bioreactor including at least two phases, i.e. a phase a) for growing said strain in the presence of at least one carbon growth substrate in...
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Zusammenfassung: | The present invention relates to a method for producing cellulases and hemicellulases by a strain belonging to a filamentous fungus, in an agitated and aerated bioreactor including at least two phases, i.e. a phase a) for growing said strain in the presence of at least one carbon growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbon growth substrate of between 10 and 90 g/L, a phase b) for the continuous production of cellulases, in which at least one carbon inducing substrate is fed at an at least constant feed rate for a period at least greater than 200 hours, said carbon substrate being at least an aqueous hemicellulose hydrolysate solution derived from an acid pretreatment of a lignocellulose substrate, said aqueous hemicellulose hydrolysate solution not being presterilized the pH thereof not being corrected, said pH of the aqueous solution being between 0.5 and 3, wherein the weight of the reaction volume is kept constant by drawing off a fraction of said reaction volume, said phase b) being carried out at a dilution rate of between 0.001 and 0.02 h-1. |
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