Integrated process for the production of oil bearing Chlorella variabilis for lipid extraction utilizing by-products of Jatropha methyl ester (JME) production

The present invention provides a simpler and more energy efficient process for the preparation of marine microalgae Chlorella fatty acid methyl ester (CME) from hydrolysate of deoiled cake of Jatropha (JOCH) and crude glycerol co-product stream (GL7 and GL8) (WO2011/027353) along with seawater dilut...

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Bibliographische Detailangaben
Hauptverfasser:	MISHRA, SANJIV KUMAR, JAIN, DEEPTI, MISHRA, SANDHYA CHANDRIKA PRASAD, UPADHYAY, SUMESH CHANDRA, MAITI, SUBARNA, PANCHA, IMRAN, ZALA, KRUSHNADEVSINH SUKHDEVSINGH, SHRIVASTAV, ANUPAMA VIJAYKUMAR, SHETHIA, BHUMI, GHOSH, PUSHPITO KUMAR, GANDHI, MAHESH RAMNIKLAL
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a simpler and more energy efficient process for the preparation of marine microalgae Chlorella fatty acid methyl ester (CME) from hydrolysate of deoiled cake of Jatropha (JOCH) and crude glycerol co-product stream (GL7 and GL8) (WO2011/027353) along with seawater diluted with tap water (1:2). A small part of the crude glycerol layer in case of JME is processed to recover glycerol for the purpose of glycerol washing and the otherwise problematic still bottom is utilized for microbial synthesis of PHAs and the rest is utilized for Microalgal conversion of JME byproducts into CME. The remaining part of the methanol- depleted glycerol layer is utilized, along with hydrolysate of the Jatropha deoiled cake(JOCH), for single-stage Microalgal production of lipids by a marine Microalgal isolate (Chlorella sp.) without the need for any other nutrients. Waste streams from the microalgal processes can be discharged directly into agricultural fields as biofertilizer or recycled back in the mass cultivation.