Continuous culture Apparatus With Mobile Vessel and Producing a Culture in a Continuous Manner

Abstract A device for growing living cells in a continuous manner, comprising: a flexible tubing containing culture medium and a surface in which the living cells can grow on in suspension or not in suspension, cohesive or not, wherein the tubing is transparent or translucent to measure turbidity; and a system of clamps, each capable of open and closed positions, the clamps being positioned so as to be able to divide the tubing into: i) an upstream region containing unused culture medium; ii) a downstream region containing spent culture medium; and iii) a growth chamber region for growing said cells disposed between the upstream and downstream regions; wherein the system of clamps is constructed and arranged to open and close so as to clamp off and define the growth chamber region of the tubing between the upstream and downstream regions of the tubing, and to cyclically redefine the growth chamber region of the tubing so that a first portion of the previously defined growth chamber region becomes a portion of the downstream region of the tubing, and a portion of the previously defined upstream region of the tubing becomes a portion of the growth chamber region of the tubing. WO 2008/013967 PCT/US2007/016960 Qi I Q QTI TF ITIE ICECT IDI II C a