Method for purifying the rabies virus

The subject matter of the invention is a method for purifying the rabies virus, comprising a single step of ion exchange chromatography, said step being a cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	FABRE, VIRGINIE, ROCCA, CELINE, RIFFARD, PIERRE, CALVOSA, ERIC
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The subject matter of the invention is a method for purifying the rabies virus, comprising a single step of ion exchange chromatography, said step being a cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography substrate comprising a matrix of polymethacrylate onto which sulphoisobutyl groups have been grafted such that the rabies virus binds to this substrate, and b) the virus is eluted from the substrate thereof.