Methods for producing secreted proteins

METHODS FOR PRODUCING SECRETED PROTEINS The present invention relates to a method for producing a heterologous protein secreted out of a plant 5 cell comprising introducing into a plant cell genome a DNA encoding an amino acid sequence that comprises a glycinin signal sequence for endoplasmic reticu...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	MATSUSHIMA, YUTAKA, NAGASAWA, AKITSU, MIGIWA, KEIKO
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	METHODS FOR PRODUCING SECRETED PROTEINS The present invention relates to a method for producing a heterologous protein secreted out of a plant 5 cell comprising introducing into a plant cell genome a DNA encoding an amino acid sequence that comprises a glycinin signal sequence for endoplasmic reticulum transport and an amino acid sequence of a heterologous protein, wherein the signal sequence is directly fused to the amino acid sequence 10 of the heterologous protein or one or two amino acids are inserted between the signal sequence and the amino acid sequence of the heterologous protein; and expressing the DNA. Fig. 7 RB Hindl[l BamHI Nhel Sac EcoRi LB NOSp NPTIl NOSt CaMV35Sp IGYlSP S Lip 1soy NOSt pBl-35S-GY1SP S-Liplsoy Fig. 8 RB Hindill BamHI Sacl EcoRi LB 44 NOSp NPTll NOSt CaMV35Sp Lip Isoy NOSt pBI-35S-Liplsoy Fig. 9 c0)4 ( 0.2 pBl-35S-GY1SP S-Lip1 soy pBI-35S-Liplsoy