Enhancing accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins

The present invention relates to improved methods for increasing accumulation levels of heterologous proteins in plant seeds for molecular farming through targeted suppression of endogenous proteins that compete against the heterologous protein for limited resources, such as amino acids, ribosomal binding sites and portfolio of enzymes participating in translation and post-translational processing. Provided are methods to suppress expression of transcription factors in barley aquired for transcriptional activation of hordein genes, using either antisense "inhibition" or double stranded RNA-induced RNA interferance or post transcriptional gene silencing (PTGS).