Novel neomycin phosphotransferase genes and method for the selection of high-producing recombinant cells

Novel neomycin phosphotransferase genes and method for the selection of high-producing recombinant cells

The invention relates to novel modified neomycin phosphotransferase genes and the use thereof in a selection method for high-producing recombinant cells. The invention also relates to expression vectors containing a modified neomycin phosphotransferase gene and a gene of interest in a functional bon...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: BARBARA ENENKEL, RALF OTTO, KERSTIN SAUTTER, KLAUS BERGEMANN, JURGEN FIEDER
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to novel modified neomycin phosphotransferase genes and the use thereof in a selection method for high-producing recombinant cells. The invention also relates to expression vectors containing a modified neomycin phosphotransferase gene and a gene of interest in a functional bond with a heterologous promoter, and a method for producing heterologous gene products by using said expression vectors.