Method and kit for pesticide analysis

A method for rapid detection of pesticide at ppt level using immunochemiluminescence is diclosed. The method comprises packing immobilized antibodies packed in a glass capillary column, passing a sample solution to be tested for pesticide content through said column so that a pesticide-antibody con...

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Bibliographische Detailangaben
Hauptverfasser:	MYSORE ANANTHARAMAIAH KUMAR, AKIMAL PASHA, MUNNA SINGH THAKUR, NITTIRU GOPALA KRISHNA KARANTH, NAIKANKATTE GANESH KARANTH, BANGALORE ESHWAR AMITA RANI
Format:	Patent
Sprache:	eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	A method for rapid detection of pesticide at ppt level using immunochemiluminescence is diclosed. The method comprises packing immobilized antibodies packed in a glass capillary column, passing a sample solution to be tested for pesticide content through said column so that a pesticide-antibody conjugate is formed, removing said pesticide-antibody conjugate from the column and adding thereto, urea H202 followed by Luminol and K3Fe(CN)6, mixing said solution thoroughly until light is generated, and capturing the light generated by a CCD camera, the intensity of the light produced being directly proportional to the amount of pesticide content in said sample. The present invention also discloses a kit for carrying out the method of the present invention.