A PROCESS FOR PREPARATION OF THERMOSTABLE ENZYME

A process for the preparation of thermostable enzyme glucose oxidase of high Transition temperature (Tm) useful for biological processes of high temperature, said process comprising steps of silanizaton of the activated glass beads by evaporative deposition of Aminopropyl triethoxysilane in acetone,...

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Bibliographische Detailangaben
Hauptverfasser:	MYSORE, ANANTHARAMAIAH KUMAR, NAIKANKATTE, GANESH KARANTH, MUNNA, SINGH THAKUR, RENU, SARATH, BABU VEGESNA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A process for the preparation of thermostable enzyme glucose oxidase of high Transition temperature (Tm) useful for biological processes of high temperature, said process comprising steps of silanizaton of the activated glass beads by evaporative deposition of Aminopropyl triethoxysilane in acetone, heating the silanated glass beads overnight at about 115°C, treating the heated glass beads with glutaraldehyde in phosphate buffer of pH about 6.0 for one hour each, both with and without vacuum, exposing the treated beads to the enzyme at about 30°C and for time duration ranging between 3-5 hours with intermittent shaking, reducing the Schiff's base obtained in preceeding step by treating the beads of step (d) with sodium cyanoborohydride, obtaining thermostable enzyme immobilized on the beads, blocking the unoccupied regions of the glass beads with glycine, and a method of using the said thermostable enzymes, and a thermostable enzyme thereof.