Plant fad2 coding sequence balancing for fatty acid profiling in edible oils

In one aspect, the invention provides novel tetraploid Brassica plants having no more than two expressible FAD2 coding sequences, capable of producing canola quality oils. Other aspects of the invention provide new variants of the FAD2 enzyme, comprising BjFAD2-b and BjFAD2-a, as well as nucleic aci...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	DARYL R. MALES, KATHERINE M. LOCKHART, DEREK A. POTTS, KENING YAO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	In one aspect, the invention provides novel tetraploid Brassica plants having no more than two expressible FAD2 coding sequences, capable of producing canola quality oils. Other aspects of the invention provide new variants of the FAD2 enzyme, comprising BjFAD2-b and BjFAD2-a, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention include nucleic acid sequences upstream from the BjFAD2-b or BjFAD2-a ORFs. Other aspects of the invention include transgenic plants and plant pads. Vectors capable of transforming plant cells are provided, comprising the nucleic acids of the invention, including FAD2 coding sequences. Corresponding methods are provided for obtaining the transgenic plants of the invention. Methods are provided for using the plants of the invention, including selected plants and transgenic plants, to obtain plant products. Amplification primers for identifying the FAD2 coding sequences of the invention are provided, together with methods of obtaining plants using the FAD2 coding sequences of the invention as markers.