Process for obtaining growth factor (TGF-BETA and IGF-1), lactoperoxidase and immunoglobulins preparations from milk products having low mutual cross-contamination

The present invention relates to a process for extracting beneficial compounds, in particular growth factors, such as TGF beta and IGF-1 from milk. In this process a hydrophobic interaction chromatography step is included. A resin having a butyl group, or a phenyl group as the ligand is used as hydr...

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Bibliographische Detailangaben
Hauptverfasser: ANDOR WILHELM JOSEPH HENDRIKS, MARINUS GERARDUS CORNELIS KIVITS, CATHARINA MARIA GALAMA
Format: Patent
Sprache:eng
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Zusammenfassung:The present invention relates to a process for extracting beneficial compounds, in particular growth factors, such as TGF beta and IGF-1 from milk. In this process a hydrophobic interaction chromatography step is included. A resin having a butyl group, or a phenyl group as the ligand is used as hydrophobic interaction resin. The resin can be eluted with a salt gradient which, when the ligand is a phenyl group, contains substantially no alcohol, and thus resulting in fractions enriched in the desired growth factors. These fractions can be separated further by means of a hydroxyapatite column.