System and method for capturing multi-color fish images

A method of obtaining multi-color FISH images is provided. The method is effected by: (a) using a color imager for capturing a dark image of a magnified biological sample stained with as least two distinct FISH probes; (b) determining dark intensity (Id) for each pixel of the dark image; (c) using t...

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Bibliographische Detailangaben
Hauptverfasser: YUVAL HARARI, OPHER SHAPIRA, ERAN KAPLAN
Format: Patent
Sprache:eng
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Zusammenfassung:A method of obtaining multi-color FISH images is provided. The method is effected by: (a) using a color imager for capturing a dark image of a magnified biological sample stained with as least two distinct FISH probes; (b) determining dark intensity (Id) for each pixel of the dark image; (c) using the color imager for capturing a fluorescently illuminated image of the magnified biological sample; and (d) correcting an intensity (I) of each pixel of the fluorescently illuminated image of the biological sample according to its dark intensity (Id) to thereby obtain a multi-color FISH image of the biological sample.