Remodeling of somatic nuclei upon addition of pluripotent cell extracts

The present invention relates to methods of producing pluripotential mammalian stem cells by reprogramming somatic cells, as well as stem cells obtained by the methods, and uses of these stem cells. In one aspect, a method of producing a stem cell from a target mammalian somatic cell involves introd...

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Bibliographische Detailangaben
Hauptverfasser:	RICHARD GREGORY WOLOWACZ, PENELOPE ANN JOHNSON
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to methods of producing pluripotential mammalian stem cells by reprogramming somatic cells, as well as stem cells obtained by the methods, and uses of these stem cells. In one aspect, a method of producing a stem cell from a target mammalian somatic cell involves introducing into the target cell a medium which includes or consists of a whole, partial or derivative extract of a reprogramming cell, wherein the extract comprises soluble components of cytoplasm and nuclear factors and wherein the extract is enriched for the nuclear factors.