TRANSGLYKOSYLIERUNGSVERFAHREN UND DIE HERSTELLUNG EINES GLYKOPROTEINS

The present invention provides a transglycosylation method and a protein catalyzing a transglycosylation reaction. A reaction of transferring a galactosyl²1†’3 N -acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosyl²1†’3 N -acetylgalactosaminyl group bound there...

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Bibliographische Detailangaben
Hauptverfasser: FUJITA, KIYOTAKA, YAMAMOTO, KENJI, OZAWA, HAYATO, KATAYAMA, TAKANE, YAMAGUCHI, MASANORI, SUZUKI, SHUNICHI
Format: Patent
Sprache:ger
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Zusammenfassung:The present invention provides a transglycosylation method and a protein catalyzing a transglycosylation reaction. A reaction of transferring a galactosyl²1†’3 N -acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosyl²1†’3 N -acetylgalactosaminyl group bound thereto at the ±-position, to a sugar chain acceptor, is carried out in the presence of a protein having an endo-±- N -acetylgalactosaminidase activity and a transglycosylation activity.