VERPACKUNG ZUR KRYOKONSERVIERUNG UND LAGERUNG VON KULTIVIERTEN GEWEBEÄQUIVALENTEN

This invention is directed to an efficient cryopreservation package design of harvested mammalian tissues and living cultured tissue equivalents made by in vitro technology. The invention involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution, agitating the...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	TONER, MEHMET, TSCHUMAKOV, ALEXANDER G, WATSON, STEPHEN R
Format:	Patent
Sprache:	ger
Schlagworte:	ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR AGRICULTURE ANIMAL HUSBANDRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES BLASTING COLD ROOMS COMBINED HEATING AND REFRIGERATION SYSTEMS CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS,e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES,DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS CONVEYING COOLING OR FREEZING APPARATUS NOT COVERED BY ANY OTHERSUBCLASS FISHING FORESTRY HANDLING THIN OR FILAMENTARY MATERIAL HEAT PUMP SYSTEMS HEATING HUMAN NECESSITIES HUNTING ICE-BOXES LIGHTING LIQUEFACTION SOLIDIFICATION OF GASES MANUFACTURE OR STORAGE OF ICE MECHANICAL ENGINEERING PACKAGES PACKAGING ELEMENTS PACKING PERFORMING OPERATIONS PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF REFRIGERATION OR COOLING REFRIGERATORS STORING TRANSPORTING TRAPPING WEAPONS
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Beschreibung
Zusammenfassung:	This invention is directed to an efficient cryopreservation package design of harvested mammalian tissues and living cultured tissue equivalents made by in vitro technology. The invention involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution, agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue, and then freezing the tissue at a very slow freezing rate. In the freezing step, extracellular ice formation is initiated by seeding. The cryopreserved tissue may be stored for indefinite periods of time prior to use. The cultured tissue equivalent is an in vitro model of the equivalent human tissue, such as skin or cornea, which, when retrieved from storage can be used for transplantation or implantation in vivo or for screening compounds in vitro.