VERFAHREN ZUM NACHWEIS VON ACETYLASE ODER DEACETYLASE AKTIVITÄT IN EINEM IMMUNOASSAY

Detection of hydrolytic enzyme activity in an immunoassay comprises incubating a dually modified luminescent labelled protein/peptide, comprising a first modified position as a substrate for an enzyme and a second modified position as an affinity enhancer for an antibody, and adding an antibody disc...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	PEIKER, CHRISTINE, KRAEMER, JOACHIM, HENCO, KARSTEN
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Detection of hydrolytic enzyme activity in an immunoassay comprises incubating a dually modified luminescent labelled protein/peptide, comprising a first modified position as a substrate for an enzyme and a second modified position as an affinity enhancer for an antibody, and adding an antibody discriminating the dually modified substrate from the enzyme generated product. The method is useful for detecting enzyme activity in an immunoassay of phosphatases PP2A and PP1 using TAMRA-Iabelled bisphosphorylated peptides, and of Histone H3 acetylase or deacetylase activity using TAMRA-labelled bisacetylated-monophosphorylated peptides. In addition the method is useful for screening modulators for enzyme activity. For the histone H3 (de)acetylase assay a bisacetylated labelled peptide can only be sufficiently discriminated by an antibody from the monoacetylated substrate if a third modified residue i.e. a phosphorylated residue is present in the peptide as affinity enhancer.