VERFAHREN UND REAGENZ ZUR BESTIMMUNG VON TRANSAMINASEN
For the determination of the transaminasae by reaction of a corresponding amino acid with alpha -ketoglutaric acid to form glutamate and the alpha -keto acid corresponding to the amino acid used, and measurement of the alpha -ketonic acid formed by reduction in the presence of lactate dehydrogenase...
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Zusammenfassung: | For the determination of the transaminasae by reaction of a corresponding amino acid with alpha -ketoglutaric acid to form glutamate and the alpha -keto acid corresponding to the amino acid used, and measurement of the alpha -ketonic acid formed by reduction in the presence of lactate dehydrogenase (LDH) and optionally of malate dehydrogenase (MDH) with NADH, forming NAD+ and alpha -oxy acid, use is made of D-LDH from lactic acid bacterium. |
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