EPITOPE VOM PRE-M/M PROTEIN AUS DEM DENGUE-VIRUS, SYNTHETISCHE PEPTIDE

The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre...

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Hauptverfasser:	CHINEA SANTIAGO, GLAY, RODRIGUEZ ROCHE, ROSMARI, PELEGRINO MARTINEZ DE LA COTERA, JOSE, PEREZ DIAZ, ANA BEATRIZ, GARAY PEREZ, HILDA ELISA, PUPO ANTUNEZ, MARITZA, GUZMAN TIRADO, GUADALUPE, REYES ACOSTA, OSVALDO, MORIER DIAZ, LUIS, GUILLEN NIETO, GERARDO ENRIQUE, PEREZ INSUITA, OMAIDA, VAZQUEZ RAMUDO, SUSANA, PADRON PALOMARES, GABRIEL, PARDO LAZO, ORLANDO LUIS, REPARTO FRAGA, ALVAREZ VERA, MAYLIN, ANASTASIO, LAWTON
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.