VERFAHREN ZUM REINIGEN BZW. ANREICHERN VON BIOLOGISCH AKTIVEN PROTEINEN UND HIERZU GEEIGNETES MITTEL

The present invention provides a process for the purification or enrichment of biologically active proteins by the addition of a selective precipitation agent, wherein an aqueous solution containing the desired protein is mixed at a pH value below its isoelectric point with such an amount of an aryl...

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Bibliographische Detailangaben
Hauptverfasser:	THUM, WALDEMAR, NAEHER, GOTTHILF, SCHEIBE, PETER, VETTER, HELLMUTH
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PROTEIN COMPOSITIONS FOR FOODSTUFFS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE WORKING-UP PROTEINS FOR FOODSTUFFS
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Beschreibung
Zusammenfassung:	The present invention provides a process for the purification or enrichment of biologically active proteins by the addition of a selective precipitation agent, wherein an aqueous solution containing the desired protein is mixed at a pH value below its isoelectric point with such an amount of an arylsulphonic acid azo dyestuff with two sulphonate groups, which are separated from one another by 8 to 10 double bonds standing in resonance relationship, among which there is present at least one N=N double bond, that at least the greater part of the biological activity of the desired active protein is present in the precipitate, the precipitate is separated off and the active protein is again brought into solution at a pH value above its isoelectric point. The present invention also provides an agent for the selective precipitation of biologically active proteins from aqueous solutions thereof without the loss of the biological activity, wherein it contains or consists of an aryl sulphonic acid azo dyestuff with two sulphonate groups, which are separated from one another by 8 to 10 double bonds standing in resonance relationship, among which there is present at least one N=N double bond.