VERFAHREN ZUR EXPRESSION HETEROLOGISCHER GENE IN DER HEFE PICHIA PASTORIS, EXPRESSIONSVEKTOREN UND TRANSFORMIERTE MIKROORGANISMEN

The present invention provides a highly efficient method for the expression of heterologous genes, using as the host an auxotrophic his3 mutant of strain BKM-90 of Pichia pastoris and as recombinant DNA for the transformation thereof an integrative vector which contains a functional selection marker...

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Hauptverfasser:	DELGADO BOADA JULIO MARCOS, FERBEYRE BINELFA GERARDO, HERRERA MARTINEZ LUIS SATURNIN, MARTINEZ SANTANA VLADIMIR, AGUIAR SANTIAGO JORGE AGUSTIN, CREMATA ALVAREZ JOSE ALBERTO, VILLAREAL BARRIOS ADELAIDA, MORALES GRILLO JUAN, MONTESINOS SEGUI RAQUEL, YONG GONZALEZ VLADIMIR, MENENDEZ ALARCUN ALFREDO, PAIFER REYES EDENA, TORRENS MADRAZO ISIS DEL CARME, SILVA RODRIGUEZ ALEJANDRO, SERALENA MENENDEZ ALINA, GONZALEZ LUPEZ TANIA CALLE L-V, MARGOLLEZ CLARK EMILIO, GONZALEZ BADILLO BEATRIZ, S OSA ESPINOSA ANGELA ESTELA
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a highly efficient method for the expression of heterologous genes, using as the host an auxotrophic his3 mutant of strain BKM-90 of Pichia pastoris and as recombinant DNA for the transformation thereof an integrative vector which contains a functional selection marker of Saccharomyces cerevisiae, the promotor of the alcohol oxidase gene (AOX1) of the yeast itself, as the transcription terminator that of glyceraldehyde 3-phosphate dehydrogenase (GAP) of S. cerevisiae, or that which is carried by the heterologous gene to be expressed, and another sequence homologous to the genome of Pichia pastoris which serves for the integration thereof. The expression vectors used further contain a heterologous gene bound to the signal peptide of the sucrose invertase gene (SUC2) of S. cerevisiae. This method guarantees that the polypeptide products are recoverable under controllable conditions and with high yields. The strains according to the present invention can be used for the large-scale production of pharmaceutical products or of enzymes of industrial interest.