VERWENDUNG EINER EXONUKLEASE UND/ODER EINER GLYKOSYLASE ALS ERGÄNZUNG ZU EINEM ANTIPOLYMERASEANTIKÖRPER FÜR DIE VERBESSERUNG DER SPEZIFITÄT DER POLYMERASEKETTENRAKTION

The present invention provides admixtures and methods for PCR amplification of a target nucleic acid in which amplification efficiency is increased by including an antibody specific for a polymerization agent and at least one of an exonuclease and a glycosylase in the PCR reaction mix. Kits for ampl...

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Bibliographische Detailangaben
Hauptverfasser: PATTERSON, DAVID R, SUTHERLAND, JOHN W. H
Format: Patent
Sprache:ger
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Beschreibung
Zusammenfassung:The present invention provides admixtures and methods for PCR amplification of a target nucleic acid in which amplification efficiency is increased by including an antibody specific for a polymerization agent and at least one of an exonuclease and a glycosylase in the PCR reaction mix. Kits for amplification of a target nucleic acid are also provided.