VERFAHREN ZUR REINIGUNG VON POLYPEPTIDEN

VERFAHREN ZUR REINIGUNG VON POLYPEPTIDEN

A process is provided for selectively separating a polypeptide of interest from components of differing hydrophobicity in a mixture comprising the steps of: (a) passing the mixture through underivatized silica particles such that the polypeptide adheres to the silica particles; (b) washing the silic...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: OLSON, CHARLES, V, REIFSNYDER, DAVID, BUILDER, STUART, E
Format: Patent
Sprache:ger
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Beschreibung
Zusammenfassung:A process is provided for selectively separating a polypeptide of interest from components of differing hydrophobicity in a mixture comprising the steps of: (a) passing the mixture through underivatized silica particles such that the polypeptide adheres to the silica particles; (b) washing the silica particles to remove impurities; and (c) eluting the polypeptide from the silica particles with a buffer comprising an alcoholic or polar aprotic solvent and an alkaline earth, an alkali metal, or an inorganic ammonium salt.