VERFAHREN ZUR GEWINNUNG VON REKOMBINANTEM HEPATITIS B VIRUS (HEP B) OBERFLÄCHEANTIGEN

A multi-step process for recovering hepatitis B surface antigen from Pichia pastoris cells containing a gene encoding said hepatitis B surface antigen and having expressed it. The process leads to a high yield of very pure, homogeneous HBsAg in a particulated form having a high immunogenicity, suita...

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Hauptverfasser:	DIAZ BETANCOURT, RAUL E, HERRERA MARTINEZ, LUIS SATURNINO, QUINONES MAYA, YAIR, PAEZ MEIRELES, ROLANDO, DUARTE CANO, CARLOS ANTONIO, PALOU, GARCIA, MANUEL, GARCIA PENA, ARNALDO, PENTON ARIAS, EDUARDO, ESQUINA ASUNCION, RAMIREZ ALBAJES, VICTORIA, GERTRUDIS Y JOSEFINA, SANTIAGO AVILA, RAMON ALEXIS, NAZABAL GALVEZ, MARCELO, DE LA RIVA DE LA RIVA, GUSTAVO ALBERTO, AGRAZ FIERRO, ALBERTO, GARCIA SUAREZ, JOSE, FONTIRROCHI ESCOBAR, GIUVEL, BELDARRAIN IZNAGA, ALEJANDRO, IZQUIERDO LOPEZ, GLADYS MABEL, AYON ZORILLA, FILIBERTO ROSENDO, PEREZ SUAREZ, LILIA LUISA, MUZIO GONZALEZ, VERENA LUCILA, PADRON GONZALEZ, GUILLERMO JULIO, GONZALEZ GRIEGO, MARTA DE JESUS, RUIZ CRUZ, CARLOS ENRIQUE
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A multi-step process for recovering hepatitis B surface antigen from Pichia pastoris cells containing a gene encoding said hepatitis B surface antigen and having expressed it. The process leads to a high yield of very pure, homogeneous HBsAg in a particulated form having a high immunogenicity, suitable for use in vaccine compositions for treating human beings and for use in diagnostic methods.